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( A ) The quantity percentage of <t>CD45</t> + , CD11b + , and MHC II + cells in control group, n = 4. ( B , C ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in DSS and LR+DSS treatment groups; n = 4 for each treatment. All samples were processed using the same gating strategy. The quantity percentage of non-viable cells is on average, 8.357 ± 0.646 (%). ( D ) The quantity percentage values of CD45 + CD11b + MHC II + cells in three groups shown with violin plot. p < 0.05 indicates a significant difference. n = 4. ( E ) The quantity percentage values of CD45 + CD11b + F4/80 − MHC II + cells in three groups, n = 4. ( F ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + cells in three groups, n = 4. ( G ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + CD64 + cells in three groups, n = 4. ( H ) Serum CRP concentration in three groups; p < 0.05 means a significant difference, n = 8.
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( A ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in control group, n = 4. ( B , C ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in DSS and LR+DSS treatment groups; n = 4 for each treatment. All samples were processed using the same gating strategy. The quantity percentage of non-viable cells is on average, 8.357 ± 0.646 (%). ( D ) The quantity percentage values of CD45 + CD11b + MHC II + cells in three groups shown with violin plot. p < 0.05 indicates a significant difference. n = 4. ( E ) The quantity percentage values of CD45 + CD11b + F4/80 − MHC II + cells in three groups, n = 4. ( F ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + cells in three groups, n = 4. ( G ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + CD64 + cells in three groups, n = 4. ( H ) Serum CRP concentration in three groups; p < 0.05 means a significant difference, n = 8.

Journal: International Journal of Molecular Sciences

Article Title: Lacticaseibacillus rhamnosus MS27 Potentially Prevents Ulcerative Colitis Through Modulation of Gut Microbiota

doi: 10.3390/ijms262311397

Figure Lengend Snippet: ( A ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in control group, n = 4. ( B , C ) The quantity percentage of CD45 + , CD11b + , and MHC II + cells in DSS and LR+DSS treatment groups; n = 4 for each treatment. All samples were processed using the same gating strategy. The quantity percentage of non-viable cells is on average, 8.357 ± 0.646 (%). ( D ) The quantity percentage values of CD45 + CD11b + MHC II + cells in three groups shown with violin plot. p < 0.05 indicates a significant difference. n = 4. ( E ) The quantity percentage values of CD45 + CD11b + F4/80 − MHC II + cells in three groups, n = 4. ( F ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + cells in three groups, n = 4. ( G ) The quantity percentage values of CD45 + CD11b + F4/80 + CD68 + CD64 + cells in three groups, n = 4. ( H ) Serum CRP concentration in three groups; p < 0.05 means a significant difference, n = 8.

Article Snippet: The CytoPeak R1 3-laser 14-color flow cytometer (Dakewe Biotech Co., Ltd., Shenzhen, China) was used to detect the CD 45 (PerCP/Cyanine5.5 Anti-Mouse CD45, Cat#E-AB-F1136J, Elabscience, Wuhan, China), CD64 (PE/Cyanine7 Anti-Mouse CD64/FcγRI Antibody[X54-5/7.1], Cat#E-AB-F1186UH, Elabscience, China), F4/80 (Elab Fluor ® Violet 610 Anti-Mouse F4/80 Antibody[CI:A3-1], Cat#E-AB-F0995T, Elabscience, China), MHC II (APC Anti-Mouse MHC II Antibody[M5/114], Cat#E-AB-F0990UE, Elabscience, China), CD206 (FITC Anti-Mouse CD206/MMR Antibody[C068C2], Cat#E-AB-F1135C, Elabscience, China), CD19 (Elab Fluor ® Violet 450 Anti-Mouse CD19 Antibody, Cat#E-AB-F0986UQ, Elabscience, China), CD11b (PE CD11b anti-mouse, Cat#E-AB-F1081D, Elabscience, China) and CD68 (CoraLite ® Plus 750 Anti-Mouse CD68 Rabbit Recombinant Antibody, Cat#CL750-98029, Proteintech, Wuhan, China).

Techniques: Control, Concentration Assay